Part:BBa_K5159014:Design

Y. lipolytica KU70 5' homology arm

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 40
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 140
  Illegal SapI.rc site found at 718
  

Design Notes

A 1000 bp homologous arm in length is necessary for the efficient knockout to occur.

Source

This part sequence is identified in the Yarrowia lipolytica genome and in our project synthesized by commercial provider GenScript.